Autoren:	Probst, Hans Christian Stoitzner, Patrizia Amon, Lukas Backer, Ronald A. Brand, Anna Chen, Jianzhou Clausen, Björn E. Dieckmann, Sophie Dudziak, Diana Heger, Lukas Hodapp, Katrin Hornsteiner, Florian Hovav, Avi-Hai Jacobi, Lukas Ji, Xingqi Kamenjarin, Nadine Lahl, Katharina Lahmar, Imran Lakus, Jelena Lehmann, Christian H. K. Ortner, Daniela Picard, Marion Roberti, Maria Paula Rossnagel, Lukas Saba, Yasmin Schalla, Carmen Schlitzer, Andreas Schraml, Barbara U. Schütze, Kristian Seichter, Anna Seré, Kristin Seretis, Athanasios Sopper, Sieghart Strandt, Helen Sykora, Martina M. Theobald, Hannah Tripp, Christoph H. Zitvogel, Laurence
Titel:	Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
Online-Publikationsdatum:	25-Jan-2023
Erscheinungsdatum:	2022
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8571
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY-NC-ND
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Zeitschrift:	European journal of immunology Version of Record (VoR)
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2022
ISSN:	1521-4141
DOI der Originalveröffentlichung:	10.1002/eji.202249819
Enthalten in den Sammlungen:	DFG-491381577-H