Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8118
Authors: | Páez-Moscoso, Diego J. Ho, David V. Pan, Lili Hildebrand, Katie Jensen, Kristi L. Levy, Michaella J. Florens, Laurence Baumann, Peter |
Title: | A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis |
Online publication date: | 27-Oct-2022 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | Telomerase reverse transcriptase (TERT) and the noncoding telomerase RNA (TR) subunit constitute the core of telomerase. Additional subunits are required for ribonucleoprotein complex assembly and in some cases remain stably associated with the active holoenzyme. Pof8, a member of the LARP7 protein family is such a constitutive component of telomerase in fission yeast. Using affinity purification of Pof8, we have identified two previously uncharacterized proteins that form a complex with Pof8 and participate in telomerase bio genesis. Both proteins participate in ribonucleoprotein complex assembly and are required for wildtype telomerase activity and telomere length maintenance. One factor we named Thc1 (Telomerase Holoenzyme Component 1) shares structural similarity with the nuclear cap binding complex and the poly-adenosine ribonuclease (PARN), the other is the ortholog of the methyl phosphate capping enzyme (Bin3/MePCE) in metazoans and was named Bmc1 (Bin3/MePCE 1) to reflect its evolutionary roots. Thc1 and Bmc1 function together with Pof8 in recognizing correctly folded telomerase RNA and promoting the recruitment of the Lsm2-8 complex and the catalytic subunit to assemble functional telomerase. |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 10 Biologie |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8118 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Nature Communications 13 |
Pages or article number: | 1067 |
Publisher: | Springer Nature |
Publisher place: | London |
Issue date: | 2022 |
ISSN: | 2041-1723 |
Publisher DOI: | 10.1038/s41467-022-28545-9 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
---|---|---|---|---|---|
a_putative_cap_binding_protei-20221020121323705.pdf | 3.94 MB | Adobe PDF | View/Open |