Gutenberg Open Science: Kinome profiling of regulatory T cells : a closer look into a complex intracellular network

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7768

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DC Field	Value	Language
dc.contributor.author	Tüttenberg, Andrea	-
dc.contributor.author	Hahn, Susanne A.	-
dc.contributor.author	Mazur, Johanna	-
dc.contributor.author	Gerhold-Ay, Aslihan	-
dc.contributor.author	Scholma, Jetse	-
dc.contributor.author	Marg, Iris	-
dc.contributor.author	Ulges, Alexander	-
dc.contributor.author	Satoh, Kazuki	-
dc.contributor.author	Bopp, Tobias	-
dc.contributor.author	Joore, Jos	-
dc.contributor.author	Jonuleit, Helmut	-
dc.date.accessioned	2022-09-15T07:42:04Z	-
dc.date.available	2022-09-15T07:42:04Z	-
dc.date.issued	2016
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7783	-
dc.description.abstract	Regulatory T cells (Treg) are essential for T cell homeostasis and maintenance of peripheral tolerance. They prevent activation of auto-reactive T effector cells (Teff) in the context of autoimmunity and allergy. Otherwise, Treg also inhibit effective immune responses against tumors. Besides a number of Treg-associated molecules such as Foxp3, CTLA-4 or GARP, known to play critical roles in Treg differentiation, activation and function, the involvement of additional regulatory elements is suggested. Herein, kinase activities seem to play an important role in Treg fine tuning. Nevertheless, our knowledge regarding the complex intracellular signaling pathways controlling phenotype and function of Treg is still limited and based on single kinase cascades so far. To gain a more comprehensive insight into the pathways determining Treg function we performed kinome profiling using a phosphorylation-based kinome array in human Treg at different activation stages compared to Teff. Here we have determined intriguing quantitative differences in both populations. Resting and activated Treg showed an altered pattern of CD28-dependent kinases as well as of those involved in cell cycle progression. Additionally, significant up-regulation of distinct kinases such as EGFR or CK2 in activated Treg but not in Teff not only resemble data we obtained in previous studies in the murine system but also suggest that those specific molecular activation patterns can be used for definition of the activation and functional state of human Treg. Taken together, detailed investigation of kinome profiles opens the possibility to identify novel molecular mechanisms for a better understanding of Treg biology but also for development of effective immunotherapies against unwanted T cell responses in allergy, autoimmunity and cancer.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Kinome profiling of regulatory T cells : a closer look into a complex intracellular network	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7768	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	PLoS one	de
jgu.journal.volume	11	de
jgu.journal.issue	2	de
jgu.pages.alternative	e0149193	de
jgu.publisher.year	2016	-
jgu.publisher.name	PLoS	de
jgu.publisher.place	Lawrence, Kan.	de
jgu.publisher.uri	http://dx.doi.org/10.1371/journal.pone.0149193	de
jgu.publisher.issn	1932-6203	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-08-23T08:18:40Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Institut für Immunologie	de_DE
opus.organisation.string	FB 04: Medizin: Institut für Med. Biometrie, Epidemologie und Informatik	de_DE
opus.organisation.string	FB 04: Medizin: Hautklinik	de_DE
opus.identifier.opusid	56315
opus.institute.number	0412
opus.institute.number	0424
opus.institute.number	0431
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Tüttenberg, Andrea
opus.affiliated	Mazur, Johanna
opus.affiliated	Gerhold-Ay, Aslihan
opus.affiliated	Bopp, Tobias
opus.affiliated	Jonuleit, Helmut
jgu.publisher.doi	10.1371/journal.pone.0149193	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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