Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-757| Authors: | Shen, Limei Tenzer, Stefan Hess, Moritz Distler, Ute Tubbe, Ingrid Montermann, Evelyn Schimmer, Simone Dittmer, Ulf Grabbe, Stephan Bros, Matthias |
| Title: | Friend virus limits adaptive cellular immune responses by imprinting a maturation-resistant and T helper type 2-biased immunophenotype in dendritic cells |
| Online publication date: | 26-Nov-2018 |
| Year of first publication: | 2018 |
| Language: | english |
| Abstract: | The murine Friend virus (FV) retrovirus model has been widely used to study anti-viral immune responses, and virus-induced cancer. Here we analyzed FV immune evasion mechanisms on the level of dendritic cells (DC) essential for the induction of primary adaptive immune responses. Comparative quantitative proteome analysis of FV-infected DC (FV-DC) of different genotypes (BALB/c, C57BL/6) and non-infected DC revealed numerous genotype-independently regulated proteins rergulating metabolic activity, cytoskeletal rearrangements, and antigen processing/presentation. These alterations may promote virion production in FV-DC. Stimulation of FV-DC with LPS resulted in strongly enhanced IL-10 production which was partially responsible for their attenuated T cell (CD4 , CD8 ) stimulatory capacity. Stimulated FV-DC induced less IFN-γ production in T cells required for cellular anti-viral responses, but more T helper cell type 2 (Th2)-associated cytokines (IL-4, IL-5, IL-13). We conclude that FV reprograms DC to promote viral spreading and immune deviation by imprinting a largely maturation-resistant, Th2-biased immunophenotype. |
| DDC: | 610 Medizin 610 Medical sciences |
| Institution: | Johannes Gutenberg-Universität Mainz |
| Department: | FB 04 Medizin |
| Place: | Mainz |
| ROR: | https://ror.org/023b0x485 |
| DOI: | http://doi.org/10.25358/openscience-757 |
| URN: | urn:nbn:de:hebis:77-publ-586564 |
| Version: | Published version |
| Publication type: | Zeitschriftenaufsatz |
| License: | CC BY |
| Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
| Journal: | PLOS ONE 13 2 |
| Pages or article number: | e0192541 |
| Publisher: | PLOS |
| Publisher place: | San Francisco, California, US |
| Issue date: | 2018 |
| ISSN: | 1932-6203 |
| Publisher URL: | http://dx.doi.org/10.1371/journal.pone.0192541 |
| Publisher DOI: | 10.1371/journal.pone.0192541 |
| Appears in collections: | JGU-Publikationen |
