Autoren:	Jäckels, Nadine Bellinghausen, Iris Fronk, Petra Heydenreich, Bärbel Saloga, Joachim Decker, Heinz
Titel:	Assessment of sensitization to grape and wine allergens as possible causes of adverse reactions to wine : a pilot study
Online-Publikationsdatum:	13-Jul-2022
Erscheinungsdatum:	2015
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	BACKGROUND: In a recently performed survey with 4000 randomly selected persons, 68 (7.2 %) of 948 respondents reported intolerance and/or allergy-like symptoms to wine. The aim of this study was to analyze whether a real sensitization to wine proteins could be confirmed by diagnostic and/or immunological settings. FINDINGS: For this purpose, 19 subjects with self-reported intolerance to wine of the invited subjects and 10 controls without a history of intolerance participated in an allergological examination (skin prick test, ImmunoCAP for determination of specific IgE antibodies, CAST for testing basophil activation, ImmunoBlot for testing specificity of IgE-antibodies). For the allergological work-up red and white grapes, selected wines, and the purified lipid transfer protein (LTP), a known grape allergen, were used. 7 subjects showed evidence of IgE sensitization to wine or grape extracts, including one control. One participant with symptoms of intolerance showed a positive skin prick test to red grape, a positive ImmunoCAP to grape, a positive cellular antigen stimulation test (CAST) and inhibition of Western blot by removal of cross-reactive carbohydrate determinants (CCD). CONCLUSION: The presented study focused on the grape protein-related IgE-mediated cause of intolerance to wine (true allergy) and not on other wine components or fining agents (other forms of intolerance). A sensitization to grape and wine proteins was observed in our cohort. In one case, this reactivity could be explained by cross-reactivity to CCD. The results of this pilot study need to be validated in greater cohorts.
DDC-Sachgruppe:	570 Biowissenschaften 570 Life sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin FB 10 Biologie
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7389
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Clinical and translational allergy 5
Seitenzahl oder Artikelnummer:	Art. 21
Verlag:	BioMed central
Verlagsort:	London
Erscheinungsdatum:	2015
ISSN:	2045-7022
URL der Originalveröffentlichung:	http://dx.doi.org/10.1186/s13601-015-0065-8
DOI der Originalveröffentlichung:	10.1186/s13601-015-0065-8
Enthalten in den Sammlungen:	DFG-OA-Publizieren (2012 - 2017)