Gutenberg Open Science: Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7382

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DC Field	Value	Language
dc.contributor.author	Hasibeder, Astrid	-
dc.contributor.author	Stein, Pamela	-
dc.contributor.author	Brandwijk, Ricardo	-
dc.contributor.author	Schild, Hansjörg	-
dc.contributor.author	Radsak, Markus	-
dc.date.accessioned	2022-07-12T09:13:04Z	-
dc.date.available	2022-07-12T09:13:04Z	-
dc.date.issued	2015
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7396	-
dc.description.abstract	Triggering receptor expressed on myeloid cells (TREM)-1 plays an important role in innate immune responses and is upregulated under infectious as well as non-infectious conditions. In addition, a soluble TREM-1 variant (sTREM-1) is detectable in sera or bronchoalveolar-lavage fluids from patients. Currently, various studies are difficult to compare, since the methods of detection by enzyme-linked immunosorbent assays (ELISA) vary among different research groups. In this study, we compared three different s-TREM-1 specific ELISAs and identified individual assay characteristics finding notable differences in sTREM-1 concentrations in part depending on the employed buffers. Investigating potential confounding factors for sTREM-1 detection, serum heat-inactivation (HI) showed improved recovery compared to non-HI (NHI) serum, reproducible by addition of complement and re-heat-inactivation. Hence we identified complement as a heat-sensitive confounder in some sTREM-1 ELISAs. We conclude that it is difficult to directly compare data of several studies, in particular if different ELISAs are engaged. Immunoassays for research use only are in general hampered by lack of standardization. Further standardization is needed until sTREM-1 ELISA is capable for better reproducibility of studies and clinical application.	de_DE
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay	fr_FR
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7382	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Scientific reports	de
jgu.journal.volume	5	de
jgu.pages.alternative	Art. 15381	de
jgu.publisher.year	2015	-
jgu.publisher.name	Nature Publ. Group	de
jgu.publisher.place	London	de
jgu.publisher.uri	http://dx.doi.org/10.1038/srep15381	de
jgu.publisher.issn	2045-2322	de
jgu.organisation.place	Mainz	-
jgu.identifier.pmid	26480887
jgu.subject.ddccode	610	de
opus.date.modified	2017-04-25T13:22:39Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Institut für Immunologie	de_DE
opus.identifier.opusid	53329
opus.importsource	pubmed
opus.institute.number	0412
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Schild, Hansjörg
opus.affiliated	Radsak, Markus
jgu.publisher.doi	10.1038/srep15381	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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