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Autoren: Manicam, Caroline
Ginter, Natalja
Li, Huige
Xia, Ning
Goloborodko, Evgeny
Zadeh, Jenia Kouchek
Musayeva, Aytan
Pfeiffer, Norbert
Gericke, Adrian
Titel: Compensatory vasodilator mechanisms in the ophthalmic artery of endothelial nitric oxide synthase gene knockout mice
Online-Publikationsdatum: 15-Jun-2022
Erscheinungsdatum: 2017
Sprache des Dokuments: Englisch
Zusammenfassung/Abstract: Nitric oxide (NO) generated by endothelial nitric oxide synthase (eNOS) plays an important role in the maintenance of ocular vascular homeostasis. Therefore, perturbations in vascular NO synthesis have been implicated in the pathogenesis of several ocular diseases. We recently reported that eNOS contributes significantly to vasodilation of the mouse ophthalmic artery. Interestingly, dilatory responses were also retained in eNOS gene-deficient mice (eNOS−/−), indicating inherent endothelial adaptive mechanism(s) that act as back-up systems in chronic absence of eNOS to preserve vasorelaxation. Thus, this study endeavoured to identify the compensatory mechanism(s) in the ophthalmic artery of eNOS−/− mice employing isolated arterial segments and pharmacological inhibitors in vitro. Endothelium removal virtually abolished acetylcholine (ACh)-induced vasodilation, suggesting an obligatory involvement of the endothelium in cholinergic control of vascular tone. However, non-NOS and non-cyclooxygenase components compensate for eNOS deficiency via endothelium-derived hyperpolarizing factors (EDHFs). Notably, arachidonic acid-derived metabolites of the 12-lipoxygenase pathway were key mediators in activating the inwardly rectifying potassium channels to compensate for chronic lack of eNOS. Conclusively, endothelium-dependent cholinergic responses of the ophthalmic artery in the eNOS−/− mice are largely preserved and, this vascular bed has the ability to compensate for the loss of normal vasodilator responses solely via EDHFs.
DDC-Sachgruppe: 610 Medizin
610 Medical sciences
Veröffentlichende Institution: Johannes Gutenberg-Universität Mainz
Organisationseinheit: FB 04 Medizin
Veröffentlichungsort: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-7153
Version: Published version
Publikationstyp: Zeitschriftenaufsatz
Nutzungsrechte: CC BY
Informationen zu den Nutzungsrechten: https://creativecommons.org/licenses/by/4.0/
Zeitschrift: Scientific reports
7
Seitenzahl oder Artikelnummer: Art. 7111
Verlag: Macmillan Publishers Limited, part of Springer Nature
Verlagsort: London
Erscheinungsdatum: 2017
ISSN: 2045-2322
URL der Originalveröffentlichung: http://dx.doi.org/10.1038/s41598-017-07768-7
DOI der Originalveröffentlichung: 10.1038/s41598-017-07768-7
Enthalten in den Sammlungen:DFG-OA-Publizieren (2012 - 2017)

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