Gutenberg Open Science: The impact of intercellular communication for the generation of complex multicellular prevascularized tissue equivalents

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7093

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DC Field	Value	Language
dc.contributor.author	Heller, Martin	-
dc.contributor.author	Bauer, Heide-Katharina	-
dc.contributor.author	Schwab, Roxana	-
dc.contributor.author	Blatt, Sebastian	-
dc.contributor.author	Peters, Katharina	-
dc.contributor.author	Nezi-Cahn, Sandra	-
dc.contributor.author	Unger, Ronald E.	-
dc.contributor.author	Hasenburg, Annette	-
dc.contributor.author	Brenner, Walburgis	-
dc.date.accessioned	2022-06-08T09:23:29Z	-
dc.date.available	2022-06-08T09:23:29Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7107	-
dc.description.abstract	In reconstructive surgery the use of prevascularized soft tissue equivalents is a promising approach for wound coverage of defects after tumor resection or trauma. However, in previous studies to generate soft tissue equivalents on collagen membranes, microcapillaries were restricted to superficial areas. In this study, to understand which factors were involved in the formation of these microcapillaries, the levels of the angiogenic factors vascular endothelial growth factor (VEGF), Interleukin-8 (IL-8), and basic fibroblast growth factor (bFGF) in the supernatants of the tissue equivalents were examined at various time points and conditions. Additionally, the influence of these factors on viability, proliferation, migration, and tube formation in monocultures compared to cocultures of fibroblast and endothelial cells was examined. The results showed that VEGF production was decreased in cocultures compared to fibroblast monocultures and the lowest VEGF levels were observed in endothelial cell monocultures. Additionally, the highest levels of IL-8 were observed in cocultures compared to monocultures. Similar results were observed for bFGF with lowest levels seen within the first 24 hr and highest levels in cocultures. VEGF and IL-8 were shown to promote endothelial cell viability, proliferation and migration and angiogenic parameters such as tube density, total tube length, and number of tube branches. Addition of VEGF and IL-8 to cocultures resulted in accelerated and denser formation of capillary-like structures. The results indicate that VEGF, IL-8, and bFGF strongly influence cellular behavior of endothelial cells and this information should be useful in promoting the formation of microcapillary-like structures in complex tissue equivalents.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	The impact of intercellular communication for the generation of complex multicellular prevascularized tissue equivalents	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7093	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Journal of biomedical materials research : A	de
jgu.journal.volume	108	de
jgu.journal.issue	3	de
jgu.pages.start	734	de
jgu.pages.end	748	de
jgu.publisher.year	2020	-
jgu.publisher.name	Wiley	de
jgu.publisher.place	New York, NY u.a.	de
jgu.publisher.issn	1552-4965	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.1002/jbm.a.36853	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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