Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-6912
Authors: | Hess, Cornelius Zeidler, Sandra Röhrich, Jörg |
Title: | Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480 |
Online publication date: | 27-Apr-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | Misuse of pregabalin and its forensic relevance is steadily increasing. The aim of this study was to evaluate the usability of the commercially available ARKTM Pregabalin II Assay (ARK Diagnostics) for serum analysis of forensic samples. Overall, 156 samples were tested by both the immunoassay and a validated liquid chromatographic–tandem mass spectrometric (LC-MS/MS) method. Sensitivity was 100%, and specificity was 98.7% (n = 79 positive cases confirmed by LC-MS/MS in a range of 380–37,000 ng/mL). A good correlation (R2 = 0.73) could also be shown between quantitative immunoassay and LC-MS/MS results. In conclusion, the assay shows excellent reliability for screening of forensic serum samples. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-6912 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Drug testing and analysis 13 6 |
Pages or article number: | 1216 1218 |
Publisher: | Wiley |
Publisher place: | Hoboken, NJ |
Issue date: | 2021 |
ISSN: | 1942-7611 |
Publisher DOI: | 10.1002/dta.2994 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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forensic_application_and_eval-20220427120613944.pdf | 91.45 kB | Adobe PDF | View/Open |