Gutenberg Open Science: IsoMAG: an automated system for the immunomagnetic isolation of squamous cell carcinoma-derived dirculating tumor dells

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6699

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DC Field	Value	Language
dc.contributor.author	Gribko, Alena	-
dc.contributor.author	Stiefel, Janis	-
dc.contributor.author	Liebetanz, Lana	-
dc.contributor.author	Nagel, Sophie Madeleine	-
dc.contributor.author	Künzel, Julian	-
dc.contributor.author	Wandrey, Madita	-
dc.contributor.author	Hagemann, Jan	-
dc.contributor.author	Stauber, Roland H.	-
dc.contributor.author	Freese, Christian	-
dc.contributor.author	Gül, Désirée	-
dc.date.accessioned	2022-01-14T11:20:25Z	-
dc.date.available	2022-01-14T11:20:25Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6709	-
dc.description.abstract	Background: detailed information about circulating tumor cells (CTCs) as an indicator of therapy response and cancer metastasis is crucial not only for basic research but also for diagnostics and therapeutic approaches. Here, we showcase a newly developed IsoMAG IMS system with an optimized protocol for fully automated immunomagnetic enrichment of CTCs, also revealing rare CTC subpopulations. Methods: using different squamous cell carcinoma cell lines, we developed an isolation protocol exploiting highly efficient EpCAM-targeting magnetic beads for automated CTC enrichment by the IsoMAG IMS system. By FACS analysis, we analyzed white blood contamination usually preventing further downstream analysis of enriched cells. Results: 1 µm magnetic beads with tosyl-activated hydrophobic surface properties were found to be optimal for automated CTC enrichment. More than 86.5% and 95% of spiked cancer cells were recovered from both cell culture media or human blood employing our developed protocol. In addition, contamination with white blood cells was minimized to about 1200 cells starting from 7.5 mL blood. Finally, we showed that the system is applicable for HNSCC patient samples and characterized isolated CTCs by immunostaining using a panel of tumor markers. Conclusion: Herein, we demonstrate that the IsoMAG system allows the detection and isolation of CTCs from HNSCC patient blood for disease monitoring in a fully-automated process with a significant leukocyte count reduction. Future developments seek to integrate the IsoMAG IMS system into an automated microfluidic-based isolation workflow to further facilitate single CTC detection also in clinical routine.	en_GB
dc.description.sponsorship	Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	IsoMAG: an automated system for the immunomagnetic isolation of squamous cell carcinoma-derived dirculating tumor dells	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6699	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Diagnostics	de
jgu.journal.volume	11	de
jgu.journal.issue	11	de
jgu.pages.alternative	2040	de
jgu.publisher.year	2021	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	https://doi.org/10.3390/diagnostics11112040	de
jgu.publisher.issn	2075-4418	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.3390/diagnostics11112040
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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