Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-6446
Authors: | Bayer, Franziska Ascher, Stefanie Kiouptsi, Klytaimnistra Kittner, Jens M. Stauber, Roland H. Reinhardt, Christoph |
Title: | Colonization with altered Schaedler flora impacts leukocyte adhesion in mesenteric ischemia-reperfusion injury |
Online publication date: | 5-Nov-2021 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | The microbiota impacts mesenteric ischemia-reperfusion injury, aggravating the interaction of leukocytes with endothelial cells in mesenteric venules. The role of defined gut microbiomes in this life-threatening pathology is unknown. To investigate how a defined model microbiome affects the adhesion of leukocytes in mesenteric ischemia-reperfusion, we took advantage of gnotobiotic isolator technology and transferred altered Schaedler flora (ASF) from C3H/HeNTac to germ-free C57BL/6J mice. We were able to detect all eight bacterial taxa of ASF in fecal samples of colonized C57BL/6J mice by PCR. Applying qRT-PCR for quantification of species-specific 16S rDNA sequences of ASF bacteria, we found a major shift in the abundance of ASF 500, which was greater in C57BL/6J mice relative to the C3H/HeNTac founder breeding pair. Using high-speed epifluorescence intravital microscopy to visualize the venules of the small bowel mesentery, we found that gnotobiotic ASF-colonized mice showed reduced leukocyte adherence, both pre- and post-ischemia. Relative to germ-free mice, the counts of adhering leukocytes were increased pre-ischemia but did not significantly increase in ASF-colonized mice in the post-ischemic state. Collectively, our results suggest a protective role of the minimal microbial consortium ASF in mesenteric ischemia-reperfusion injury. |
DDC: | 570 Biowissenschaften 570 Life sciences 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-6446 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Microorganisms 9 8 |
Pages or article number: | 1601 |
Publisher: | MDPI |
Publisher place: | Basel |
Issue date: | 2021 |
ISSN: | 2076-2607 |
Publisher URL: | https://doi.org/10.3390/microorganisms9081601 |
Publisher DOI: | 10.3390/microorganisms9081601 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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bayer_franziska-colonization_w-20211102131705502.pdf | 1.3 MB | Adobe PDF | View/Open |