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http://doi.org/10.25358/openscience-6027Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Placentino, Maria | - |
| dc.contributor.author | Jesus Domingues, António Miguel de | - |
| dc.contributor.author | Schreier, Jan | - |
| dc.contributor.author | Dietz, Sabrina | - |
| dc.contributor.author | Hellmann, Svenja | - |
| dc.contributor.author | Albuquerque, Bruno F. M. de | - |
| dc.contributor.author | Butter, Falk | - |
| dc.contributor.author | Ketting, René F. | - |
| dc.date.accessioned | 2021-06-28T08:35:28Z | - |
| dc.date.available | 2021-06-28T08:35:28Z | - |
| dc.date.issued | 2021 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/6036 | - |
| dc.description.abstract | In Caenorhabditis elegans, the piRNA (21U RNA) pathway is required to establish proper gene regulation and an immortal germline. To achieve this, PRG-1-bound 21U RNAs trigger silencing mechanisms mediated by RNA-dependent RNA polymerase (RdRP)-synthetized 22G RNAs. This silencing can become PRG-1-independent and heritable over many generations, a state termed RNA-induced epigenetic gene silencing (RNAe). How and when RNAe is established, and how it is maintained, is not known. We show that maternally provided 21U RNAs can be sufficient for triggering RNAe in embryos. Additionally, we identify PID-2, a protein containing intrinsically disordered regions (IDRs), as a factor required for establishing and maintaining RNAe. PID-2 interacts with two newly identified and partially redundant eTudor domain-containing proteins, PID-4 and PID-5. PID-5 has an additional domain related to the X-prolyl aminopeptidase APP-1, and binds APP-1, implicating potential N-terminal proteolysis in RNAe. All three proteins are required for germline immortality, localize to perinuclear foci, affect size and appearance of RNA inheritance-linked Z granules, and are required for balancing of 22G RNA populations. Overall, our study identifies three new proteins with crucial functions in C. elegans small RNA silencing. | en_GB |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 570 Biowissenschaften | de_DE |
| dc.subject.ddc | 570 Life sciences | en_GB |
| dc.title | Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-6027 | - |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 10 Biologie | de |
| jgu.organisation.number | 7970 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | The EMBO journal | de |
| jgu.journal.volume | 40 | de |
| jgu.pages.alternative | e105280 | de |
| jgu.publisher.year | 2021 | - |
| jgu.publisher.name | Wiley | de |
| jgu.publisher.place | Hoboken, NJ u.a. | de |
| jgu.publisher.uri | https://doi.org/10.15252/embj.2020105280 | de |
| jgu.publisher.issn | 1460-2075 | de |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 570 | de |
| jgu.publisher.doi | 10.15252/embj.2020105280 | |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| Appears in collections: | JGU-Publikationen | |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | placentino_maria-intrinsically_-20210607181556177.pdf | 3.28 MB | Adobe PDF | View/Open |