Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
http://doi.org/10.25358/openscience-5782
Autoren: | Blatt, Sebastian Pabst, Andreas Zimmer, Stefanie Walter, Christian Al-Nawas, Bilal Krüger, Maximilian |
Titel: | Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
Online-Publikationsdatum: | 3-Mai-2021 |
Erscheinungsdatum: | 2021 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | OBJECTIVES There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. MATERIALS AND METHODS In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. RESULTS Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. CONCLUSION The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. CLINICAL RELEVANCE L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. |
DDC-Sachgruppe: | 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-5782 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/4.0/ |
Zeitschrift: | Clinical oral investigations 25 |
Seitenzahl oder Artikelnummer: | 2837 2843 |
Verlag: | Springer |
Verlagsort: | Berlin u.a. |
Erscheinungsdatum: | 2021 |
ISSN: | 1436-3771 |
URL der Originalveröffentlichung: | https://doi.org/10.1007/s00784-020-03601-0 |
DOI der Originalveröffentlichung: | 10.1007/s00784-020-03601-0 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
---|---|---|---|---|---|
blatt_sebastian-clinical_effic-20210503120346359.pdf | 706.44 kB | Adobe PDF | Öffnen/Anzeigen |