Gutenberg Open Science: In-depth immune-oncology studies of the tumor microenvironment in a humanized melanoma mouse model

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5612

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DC Field	Value	Language
dc.contributor.author	Schupp, Jonathan	-
dc.contributor.author	Christians, Arne	-
dc.contributor.author	Zimmer, Niklas	-
dc.contributor.author	Gleue, Lukas	-
dc.contributor.author	Jonuleit, Helmut	-
dc.contributor.author	Helm, Mark	-
dc.contributor.author	Tüttenberg, Andrea	-
dc.date.accessioned	2021-11-09T08:07:32Z	-
dc.date.available	2021-11-09T08:07:32Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5616	-
dc.description.abstract	The presence and interaction of immune cells in the tumor microenvironment is of significant importance and has a great impact on disease progression and response to therapy. Hence, their identification is of high interest for prognosis and treatment decisions. Besides detailed phenotypic analyses of immune, as well as tumor cells, spatial analyses is an important parameter in the complex interplay of neoplastic and immune cells—especially when moving into focus efforts to develop and validate new therapeutic strategies. Ex vivo analysis of tumor samples by immunohistochemistry staining methods conserves spatial information is restricted to single markers, while flow cytometry (disrupting tissue into single cell suspensions) provides access to markers in larger numbers. Nevertheless, this comes at the cost of scarifying morphological information regarding tissue localization and cell–cell contacts. Further detrimental effects incurred by, for example, tissue digestion include staining artifacts. Consequently, ongoing efforts are directed towards methods that preserve, completely or in part, spatial information, while increasing the number of markers that can potentially be interrogated to the level of conventional flow cytometric methods. Progression in multiplex immunohistochemistry in the last ten years overcame the limitation to 1–2 markers in classical staining methods using DAB with counter stains or even pure chemical staining methods. In this study, we compared the multiplex method Chipcytometry to flow cytometry and classical IHCP using DAB and hematoxylin. Chipcytometry uses frozen or paraffin-embedded tissue sections stained with readily available commercial fluorophore-labeled antibodies in repetitive cycles of staining and bleaching. The iterative staining approach enables sequential analysis of a virtually unlimited number of markers on the same sample, thereby identifying immune cell subpopulations in the tumor microenvironment in the present study in a humanized mouse melanoma model. Keywords: Chipcytometry multiplex immunohistochemistry melanoma humanized mice tumor microenvironment flow cytometry	en_GB
dc.description.sponsorship	Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	540 Chemie	de_DE
dc.subject.ddc	540 Chemistry and allied sciences	en_GB
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	In-depth immune-oncology studies of the tumor microenvironment in a humanized melanoma mouse model	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5612	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	International journal of molecular sciences	de
jgu.journal.volume	22	de
jgu.journal.issue	3	de
jgu.pages.alternative	1011	de
jgu.publisher.year	2021	-
jgu.publisher.name	Molecular Diversity Preservation International	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	https://doi.org/10.3390/ijms22031011	de
jgu.publisher.issn	1422-0067	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	540	de
jgu.subject.ddccode	570	de
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.3390/ijms22031011
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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