Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5492
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLagrange, Jeremy-
dc.contributor.authorFinger, Stefanie-
dc.contributor.authorKossmann, Sabine-
dc.contributor.authorGarlapati, Venkata-
dc.contributor.authorRuf, Wolfram-
dc.contributor.authorWenzel, Philip-
dc.date.accessioned2020-12-11T11:09:08Z-
dc.date.available2020-12-11T11:09:08Z-
dc.date.issued2020-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/5496-
dc.description.abstractMyeloid cells are crucial for the development of vascular inflammation. Low-density lipoprotein receptor-related protein 8 (LRP8) or Apolipoprotein E receptor 2 (ApoER2), is expressed by macrophages, endothelial cells and platelets and has been implicated in the development of cardiovascular diseases. Our aim was to evaluate the role of LRP8, in particular from immune cells, in the development of vascular inflammation. Methods. LRP8+/+ and LRP8−/− mice (on B6;129S background) were infused with angiotensin II (AngII, 1 mg/kg/day for 7 to 28 day) using osmotic minipumps. Blood pressure was recorded using tail cuff measurements. Vascular reactivity was assessed in isolated aortic segments. Leukocyte activation and infiltration were assessed by flow cytometry of aortic tissue and intravital videomicroscopy imaging. Histological analysis of aortic sections was conducted using sirius red staining. Results. AngII infusion worsened endothelial-dependent vascular relaxation and immune cells rolling and adherence to the carotid artery in both LRP8+/+ as well as LRP8−/− mice. However, only LRP8−/− mice demonstrated a drastically increased mortality rate in response to AngII due to aortic dissection. Bone marrow transplantation revealed that chimeras with LRP8 deficient myeloid cells phenocopied LRP8−/− mice. Conclusion. AngII-infused LRP8 deficient mice could be a useful animal model to study aortic dissection reflecting the lethality of this disease in humans. Keywords: low-density lipoprotein receptor-related protein 8 angiotensin II aortic dissectionen_GB
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainzde
dc.language.isoengde
dc.rightsCC BYde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleAngiotensin II infusion leads to aortic dissection in LRP8 deficient miceen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-5492-
jgu.type.contenttypeScientific articlede
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleInternational journal of molecular sciencesde
jgu.journal.volume21de
jgu.journal.issue14de
jgu.pages.alternative4916de
jgu.publisher.year2020-
jgu.publisher.nameMDPIde
jgu.publisher.placeBaselde
jgu.publisher.urihttps://doi.org/10.3390/ijms21144916de
jgu.publisher.issn1422-0067de
jgu.organisation.placeMainz-
jgu.subject.ddccode540de
jgu.subject.ddccode570de
jgu.subject.ddccode610de
jgu.publisher.doi10.3390/ijms21144916
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

Files in This Item:
  File Description SizeFormat
Thumbnail
lagrange_jeremy-angiotensin_ii-20201211100739818.pdf1.88 MBAdobe PDFView/Open