Gutenberg Open Science: Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5093

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DC Field	Value	Language
dc.contributor.author	Lauer, Alina	-
dc.contributor.author	Wolf, Philipp	-
dc.contributor.author	Mehler, Dorothea	-
dc.contributor.author	Götz, Hermann	-
dc.contributor.author	Rüzgar, Mehmet	-
dc.contributor.author	Baranowski, Andreas	-
dc.contributor.author	Henrich, Dirk	-
dc.contributor.author	Rommens, Pol Maria	-
dc.contributor.author	Ritz, Ulrike	-
dc.date.accessioned	2020-08-31T07:52:10Z	-
dc.date.available	2020-08-31T07:52:10Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5097	-
dc.description.abstract	Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration. Keywords: bone tissue regeneration; 3D printed cell-free scaffold; polylactide; collagen type I; stromal-derived factor 1; in vivo model of critical size defects	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	de_DE
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	-
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5093	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	International journal of molecular sciences	de
jgu.journal.volume	21	de
jgu.journal.issue	6	de
jgu.pages.alternative	2175	de
jgu.publisher.year	2020	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	https://doi.org/10.3390/ijms21062175	de
jgu.publisher.issn	1422-0067	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.3390/ijms21062175
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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