Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-5093
Authors: | Lauer, Alina Wolf, Philipp Mehler, Dorothea Götz, Hermann Rüzgar, Mehmet Baranowski, Andreas Henrich, Dirk Rommens, Pol Maria Ritz, Ulrike |
Title: | Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration |
Online publication date: | 31-Aug-2020 |
Year of first publication: | 2020 |
Language: | english |
Abstract: | Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration. Keywords: bone tissue regeneration; 3D printed cell-free scaffold; polylactide; collagen type I; stromal-derived factor 1; in vivo model of critical size defects |
DDC: | 570 Biowissenschaften 570 Life sciences 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-5093 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | International journal of molecular sciences 21 6 |
Pages or article number: | 2175 |
Publisher: | MDPI |
Publisher place: | Basel |
Issue date: | 2020 |
ISSN: | 1422-0067 |
Publisher URL: | https://doi.org/10.3390/ijms21062175 |
Publisher DOI: | 10.3390/ijms21062175 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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lauer_alina-biofabrication-20200820174943030.pdf | 4.19 MB | Adobe PDF | View/Open |