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http://doi.org/10.25358/openscience-4646| Authors: | Croxford, Andrew Lewis |
| Title: | Novel mouse models for use in IL-17A and Th17 research |
| Online publication date: | 29-Jul-2010 |
| Year of first publication: | 2010 |
| Language: | english |
| Abstract: | Th17 cells have emerged as a proinflamatory cell type with strong links to autoimmunity and immunopathology. The aims of this thesis are two-fold; Firstly, generation of a novel mouse model that allows in vivo and/or ex vivo observation and manipulation of Th17 cells. Secondly, to generate a mouse model capable of conditionally overexpressing the hallmark Th17 cytokine, IL-17A. Given the expertise and experience in our lab with respect to conditional gene targeting, Cre-LoxP-mediated approaches were chosen and utilized to achieve this goal in both mouse models. The resulting strains and the knowledge generated from their useage are discussed in this work. Furthermore, the recently
generated IL-6Rα conditional allele allows for ablation of IL-6 signaling in a cell type-specific manner. We wanted to analyze the role of IL-6 signaling with respect to EAE pathogenesis and development of pathogenic Th17 cells, and the results generated are published in this work. Andrew Lewis Croxford Zusammenfassung Th17 Zellen sind pathogenische Zellen, die mit diverse Autoimmun-Krankheiten verbunden sind. In dieser Doktorarbeit wollten wir zwei Ziele erreichen: Erstens, die Herstullung eines Mausmodels, das die Untersuchung von IL-17A-vermittelten Entzündungen ermöglicht. Zweitens, ein Mausmodel, in dem Th17 Zellen durch Fluorezenz zu erkennen sind. In unserem Labor haben wir die Erfahrung, solche Mausstämme zu generieren. Wir haben einen Ansatz ausgewählt, in dem wir die Cre-LoxP Technik verwenden, um IL-17A zu überexprimieren. Weiterhin möchten wir das neu-generierte IL-6R konditionelle Allele benutzen, um die Rolle der IL-6 Signale bei der Entwicklung von Th17 Zellen zu erforschen. Alle Ergebnisse sind in dieser Arbeit veröffentlicht worden. |
| DDC: | 570 Biowissenschaften 570 Life sciences |
| Institution: | Johannes Gutenberg-Universität Mainz |
| Department: | FB 10 Biologie |
| Place: | Mainz |
| ROR: | https://ror.org/023b0x485 |
| DOI: | http://doi.org/10.25358/openscience-4646 |
| URN: | urn:nbn:de:hebis:77-23389 |
| Version: | Original work |
| Publication type: | Dissertation |
| License: | In Copyright |
| Information on rights of use: | https://rightsstatements.org/vocab/InC/1.0/ |
| Appears in collections: | JGU-Publikationen |
