Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-24
Authors: | Funke, Sebastian Beutgen, Vanessa M. Bechter, Lea Schmelter, Carsten Zurawski, Vanessa Perumal, Natarajan Pfeiffer, Norbert Grus, Franz-Hermann |
Title: | An in-depth view of the porcine trabecular meshwork proteome |
Online publication date: | 27-Jan-2020 |
Year of first publication: | 2019 |
Language: | english |
Abstract: | The house swine (Sus scrofa domestica Linnaeus 1758) is an important model organism regarding the study of neurodegenerative diseases, especially ocular neuropathies such as glaucoma. This is due to the high comparability of the porcine and human eye regarding anatomy and molecular features. In the pathogenesis of glaucoma, the trabecular meshwork (TM) forms a key ocular component in terms of intraocular pressure (IOP) elevation. Thereby, functional TM abnormalities are correlated with distinct proteomic alterations. However, a detailed analysis of the TM proteome has not been realized so far. Since the porcine eye has high potential as a model system to study ocular diseases such as glaucoma, the present study focuses on the in-depth analysis of the porcine TM proteome. By use of a bottom-up (BU) mass spectrometric (MS) platform utilizing electrospray ionization liquid chromatography tandem MS (LC-ESI-MS/MS) considering database-dependent and peptide de novo sequencing, more than 3000 TM proteins were documented with high confidence (FDR < 1%). A distinct number of proteins with neuronal association were revealed. To the best to our knowledge, many of these protein species have not been reported for TM tissue before such as reelin, centlein and high abundant neuroblast differentiation-associated protein AHNAK (AHNAK). Thereby, AHNAK might play a superordinate role in the TM regarding proposed tissue involvement in barrier function. Also, a high number of secretory proteins could be identified. The generated TM proteomic landscape underlines a multifunctional character of the TM beyond representing a simple drainage system. Finally, the protein catalogue of the porcine TM provides an in-depth view of the TM molecular landscape and will serve as an important reference map in terms of glaucoma research utilizing porcine animal models, porcine TM tissues and/or cultured TM cells. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-24 |
URN: | urn:nbn:de:hebis:77-publ-595234 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | International journal of molecular sciences 20 10 |
Pages or article number: | Art. 2526 |
Publisher: | Molecular Diversity Preservation International |
Publisher place: | Basel |
Issue date: | 2019 |
ISSN: | 1422-0067 1661-6596 |
Publisher URL: | http://dx.doi.org/10.3390/ijms20102526 |
Publisher DOI: | 10.3390/ijms20102526 |
Appears in collections: | JGU-Publikationen |