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http://doi.org/10.25358/openscience-254
Autoren: | Khandagale, Avinash Kittner, Jens M. Mann, Amrit Ascher, Stefanie Kollar, Bettina Reinhardt, Christoph |
Titel: | Coagulation factor 9-deficient mice are protected against dextran sulfate sodium-induced colitis |
Online-Publikationsdatum: | 19-Dez-2018 |
Erscheinungsdatum: | 2018 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Patients with inflammatory bowel disease (IBD) are susceptible to thromboembolism. Interestingly, IBD occurs less frequently in patients with inherited bleeding disorders. Therefore, we analyzed whether F9-deficiency is protective against the onset of acute colitis in a genetic hemophilia B mouse model. In the 3.5% dextran sulfate sodium (DSS)-induced colitis model, F9 deficient mice were protected from body-weight loss and had a reduced disease activity score. We detected decreased colonic myeloperoxidase activity and decreased CXCL1 levels in DSS-treated F9 deficient mice compared with wild-type (WT) littermate controls, indicating decreased neutrophil infiltration. Remarkably, we identified expression of coagulation factor IX (FIX) protein in small intestinal epithelial cells (MODE-K). In epithelial cell cultures, cellular FIX protein expression was increased following stimulation with the bacterial Toll-like receptor agonists lipopolysaccharide, macrophage-activating lipopeptide-2 and Pam3CSK4. Thus, we revealed a protective role of F9-deficiency in DSS-induced colitis and identified the intestinal epithelium as a site of ectopic FIX.This article has an associated First Person interview with the first author of the paper. |
DDC-Sachgruppe: | 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-254 |
URN: | urn:nbn:de:hebis:77-publ-587104 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/3.0/ |
Zeitschrift: | Biology open 7 7 |
Seitenzahl oder Artikelnummer: | bio034140 |
Verlag: | Company |
Verlagsort: | Cambridge |
Erscheinungsdatum: | 2018 |
ISSN: | 2046-6390 |
URL der Originalveröffentlichung: | http://dx.doi.org/10.1242/bio.034140 |
DOI der Originalveröffentlichung: | 10.1242/bio.034140 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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58710.pdf | 1.01 MB | Adobe PDF | Öffnen/Anzeigen |