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Autoren: Manicam, Caroline
Perumal, Natarajan
Wasielica-Poslednik, Joanna
Ngongkole, Yong Cajetan
Tschäbunin, Alexandra
Sievers, Marcel
Lisch, Walter
Pfeiffer, Norbert
Grus, Franz-Hermann
Gericke, Adrian
Titel: Proteomics unravels the regulatory mechanisms in human tears following acute renouncement of contact lens use : a comparison between hard and soft lenses
Online-Publikationsdatum: 19-Dez-2018
Erscheinungsdatum: 2018
Sprache des Dokuments: Englisch
Zusammenfassung/Abstract: Contact lenses (CLs) provide a superior alternative to spectacles. Although beneficial, the global burden of ocular dysfunctions attributed to regular use of CLs remains a topic of much challenge in ophthalmic research owing to debilitating clinical repercussions on the ocular surface, which are often manifested as breach in tear film integrity. This study elucidated the intricate tear proteome changes attributed to the use of different CLs (hard and soft) and unravelled, for the first time, the restorative mechanisms of several protein clusters following acute renouncement of CL use employing the label-free mass spectrometry-based quantitative proteomics approach. The expression patterns of certain proteins clusters were specific to the use of a particular lens type and a large majority of these actively regulates cell death and survival and, modulates cellular movement on the ocular surface. Noteworthy, CL use also evoked a significant upregulation of glycolytic enzymes associated with hypoxia and corresponding cognate metabolic pathways, particularly glucose metabolism and FXR/RXR pathways. Importantly, the assessment of CL renouncement unravelled the restorative properties of several clusters of proteins involved mainly in organismal injury and abnormalities and, cellular function and maintenance. These proteins play key roles in restoring tear homeostasis and wound-healing mechanisms post-CL use-elicited injury.
DDC-Sachgruppe: 610 Medizin
610 Medical sciences
Veröffentlichende Institution: Johannes Gutenberg-Universität Mainz
Organisationseinheit: FB 04 Medizin
Veröffentlichungsort: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-253
Version: Published version
Publikationstyp: Zeitschriftenaufsatz
Nutzungsrechte: CC BY
Informationen zu den Nutzungsrechten: https://creativecommons.org/licenses/by/4.0/
Zeitschrift: Scientific reports
8
Seitenzahl oder Artikelnummer: Art. 11526
Verlag: Macmillan Publishers Limited, part of Springer Nature
Verlagsort: London
Erscheinungsdatum: 2018
ISSN: 2045-2322
URL der Originalveröffentlichung: http://dx.doi.org/10.1038/s41598-018-30032-5
DOI der Originalveröffentlichung: 10.1038/s41598-018-30032-5
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