Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-172
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dc.contributor.authorBeutgen, Vanessa M.-
dc.contributor.authorPerumal, Natarajan-
dc.contributor.authorPfeiffer, Norbert-
dc.contributor.authorGrus, Franz-Hermann-
dc.date.accessioned2019-07-10T09:55:05Z-
dc.date.available2019-07-10T11:55:05Z-
dc.date.issued2019-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/174-
dc.description.abstractGlaucoma is an optic neurological disorder and the leading cause of irreversible blindness worldwide worldwide, with primary open angle glaucoma (POAG) as its most prevalent form. An early diagnosis of of the disease is crucial to prevent loss of vision. Mechanisms behind glaucoma pathogenesis are not completely understood, but disease related alterations in the serological autoantibody profile indicate indicate an immunologic component. These changes in immunoreactivity may serve as potential biomarkers biomarkers for glaucoma diagnostics. We aimed to identify novel disease related autoantibodies targeting targeting antigens in the trabecular meshwork as biomarkers to support early detection of POAG. We used used serological proteome analysis (SERPA) for initial autoantibody profiling in a discovery sample set set. The identified autoantibodies were validated by protein microarray analysis in a larger cohort with with 60 POAG patients and 45 control subjects. In this study, we discovered CALD1, PGAM1 and VDAC2 as as new biomarker candidates. With the use of artificial neural networks, the panel of these candidates candidates and the already known markers HSPD1 and VIM was able to classify subjects into POAG patients patients and healthy controls with a sensitivity of 81% and a specificity of 93%. These results suggest suggest the benefit of these potential autoantibody biomarkers in utilization in glaucoma diagnostics diagnostics.en_GB
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin-
dc.language.isoeng-
dc.rightsCC BYde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleAutoantibody biomarker discovery in primary open angle glaucoma using serological proteome analysis (SERPA)en_GB
dc.typeZeitschriftenaufsatzde_DE
dc.identifier.urnurn:nbn:de:hebis:77-publ-591413-
dc.identifier.doihttp://doi.org/10.25358/openscience-172-
jgu.type.dinitypearticle-
jgu.type.versionPublished versionen_GB
jgu.type.resourceText-
jgu.organisation.departmentFB 04 Medizin-
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleFrontiers in immunology-
jgu.journal.volume10-
jgu.pages.alternativeArt. 381-
jgu.publisher.year2019-
jgu.publisher.nameFrontiers Media-
jgu.publisher.placeLausanne-
jgu.publisher.urihttp://dx.doi.org/10.3389/fimmu.2019.00381-
jgu.publisher.issn1664-3224-
jgu.organisation.placeMainz-
jgu.subject.ddccode610-
opus.date.accessioned2019-07-10T09:55:05Z-
opus.date.modified2019-08-06T08:55:50Z-
opus.date.available2019-07-10T11:55:05-
opus.subject.dfgcode00-000-
opus.organisation.stringFB 04: Medizin: Augenklinik und Poliklinikde_DE
opus.identifier.opusid59141-
opus.institute.number0446-
opus.metadataonlyfalse-
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_GB
opus.affiliatedPfeiffer, Norbert-
opus.affiliatedGrus, Franz-Hermann-
jgu.publisher.doi10.3389/fimmu.2019.00381
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

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