Autoren:	Beutgen, Vanessa M. Perumal, Natarajan Pfeiffer, Norbert Grus, Franz-Hermann
Titel:	Autoantibody biomarker discovery in primary open angle glaucoma using serological proteome analysis (SERPA)
Online-Publikationsdatum:	10-Jul-2019
Erscheinungsdatum:	2019
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Glaucoma is an optic neurological disorder and the leading cause of irreversible blindness worldwide worldwide, with primary open angle glaucoma (POAG) as its most prevalent form. An early diagnosis of of the disease is crucial to prevent loss of vision. Mechanisms behind glaucoma pathogenesis are not completely understood, but disease related alterations in the serological autoantibody profile indicate indicate an immunologic component. These changes in immunoreactivity may serve as potential biomarkers biomarkers for glaucoma diagnostics. We aimed to identify novel disease related autoantibodies targeting targeting antigens in the trabecular meshwork as biomarkers to support early detection of POAG. We used used serological proteome analysis (SERPA) for initial autoantibody profiling in a discovery sample set set. The identified autoantibodies were validated by protein microarray analysis in a larger cohort with with 60 POAG patients and 45 control subjects. In this study, we discovered CALD1, PGAM1 and VDAC2 as as new biomarker candidates. With the use of artificial neural networks, the panel of these candidates candidates and the already known markers HSPD1 and VIM was able to classify subjects into POAG patients patients and healthy controls with a sensitivity of 81% and a specificity of 93%. These results suggest suggest the benefit of these potential autoantibody biomarkers in utilization in glaucoma diagnostics diagnostics.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-172
URN:	urn:nbn:de:hebis:77-publ-591413
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Frontiers in immunology 10
Seitenzahl oder Artikelnummer:	Art. 381
Verlag:	Frontiers Media
Verlagsort:	Lausanne
Erscheinungsdatum:	2019
ISSN:	1664-3224
URL der Originalveröffentlichung:	http://dx.doi.org/10.3389/fimmu.2019.00381
DOI der Originalveröffentlichung:	10.3389/fimmu.2019.00381
Enthalten in den Sammlungen:	JGU-Publikationen