Autoren:	Mikuličić, Snježana Finke, Jérôme Boukhallouk, Fatima Wüstenhagen, Elena Sons, Dominik Homsi, Yahya Reiss, Karina Lang, Thorsten Florin, Luise
Titel:	ADAM17-dependent signaling is required for oncogenic human papillomavirus entry platform assembly
Online-Publikationsdatum:	8-Jul-2019
Erscheinungsdatum:	2019
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry include the tetraspanin CD151 and the epidermal growth factor receptor (EGFR). To date, it is unknown how these components are coordinated in space and time. Here, we studied plasma membrane dynamics of CD151 and EGFR and the HPV16 capsid during the early phase of infection. We find that the proteinase ADAM17 activates the extracellular signal-regulated kinases (ERK1/2) pathway by the shedding of growth factors which triggers the formation of an endocytic entry platform. Infectious endocytic entry platforms carrying virus particles consist of two-fold larger CD151 domains containing the EGFR. Our finding clearly dissects initial virus binding from ADAM17-dependent assembly of a HPV/CD151/EGFR entry platform.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-166
URN:	urn:nbn:de:hebis:77-publ-591345
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	eLife 8
Seitenzahl oder Artikelnummer:	e44345
Verlag:	eLife Sciences Publications
Verlagsort:	Cambridge
Erscheinungsdatum:	2019
ISSN:	2050-084X
URL der Originalveröffentlichung:	http://dx.doi.org/10.7554/eLife.44345.001
DOI der Originalveröffentlichung:	10.7554/eLife.44345.001
Enthalten in den Sammlungen:	JGU-Publikationen