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Lydia Tome-Stangl, Cornelia Schaetzel, Stefan Tenzer, Frank Bernhard, Dirk Schneider
The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6
www.plosone.org
www.plosone.org
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-34.2819 -1.2983 Td
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1.0205 0 Td
(the)Tj
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1.9445 0 Td
(type)Tj
1.9106 0 Td
(\(wt\))Tj
1.8821 0 Td
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3.1578 0 Td
([)Tj
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(17)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(20)Tj
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(].)Tj
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1.0148 0 Td
(a)Tj
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(helix-loop-helix)Tj
6.5593 0 Td
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(remotely)Tj
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1.4683 0 Td
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(22)Tj
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(24)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 460.5732 263.7354 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(6)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 466.3559 265.3795 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(\(cyt.)Tj
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(b)Tj
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Q
q
1 j
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7.4999 0 0 6.6659 491.0173 263.7354 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(6)Tj
ET
Q
q
1 j
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0 w
10 0 0 10 494.5889 265.3795 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(\).)Tj
0.788 0 Td
(Cyt.)Tj
1.8595 0 Td
(b)Tj
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Q
q
1 j
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7.4999 0 0 6.6659 526.1669 263.7354 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 531.8929 265.3795 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(a)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 278.7023 250.7527 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 282.274 252.3968 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(complex)Tj
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([)Tj
0.83 0.64 0.02 0 k
(25)Tj
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-34.7524 -1.3039 Td
(27)Tj
0 g
(].)Tj
1.7348 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 273.6566 211.748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 279.4393 213.3921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(formed)Tj
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Q
q
1 j
1 J
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7.4999 0 0 6.6659 307.6724 185.7259 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 313.3984 187.4267 cm
BT
/F7 1 Tf
1 TL
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([)Tj
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(8)Tj
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(28)Tj
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Q
q
1 j
1 J
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7.4999 0 0 6.6659 388.1196 185.7259 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(559)Tj
ET
Q
q
1 j
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BT
/F7 1 Tf
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([)Tj
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(9)Tj
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[()12()]TJ
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[()-13()14()7()20()]TJ
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[()12()]TJ
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[()-13()15()3()]TJ
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[()12()]TJ
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[()-13()14()7()20()]TJ
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ET
Q
q
1 j
1 J
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7.4999 0 0 6.6659 372.4724 159.7606 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(6)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 378.1984 161.4047 cm
BT
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-0.004 Tc
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(redox)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 243.8929 146.7212 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(L)Tj
ET
Q
q
1 j
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BT
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1.7348 0 Td
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Q
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1 j
1 J
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7.4999 0 0 6.6659 409.6629 146.7212 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(H)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 415.3889 148.422 cm
BT
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 403.8236 133.7385 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 409.6062 135.3826 cm
BT
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-0.004 Tc
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(isolated)Tj
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3.2995 0 Td
(thylakoid)Tj
3.9798 0 Td
(mem-)Tj
-33.7263 -1.2982 Td
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2.863 0 Td
([)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(])Tj
1.8538 0 Td
(and)Tj
1.7292 0 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 285.7322 120.7559 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 291.5149 122.4 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(embedded)Tj
4.3993 0 Td
(within)Tj
2.8119 0 Td
(the)Tj
/F12 1 Tf
1.474 0 Td
[( )-4(\n)-3()13()6()2()-9()4()13()18()4(\r)]TJ
4.6262 0 Td
[()7()10()12()]TJ
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1.559 0 Td
(\()Tj
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[( )]TJ
/F7 1 Tf
(.)Tj
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[()7()10()12()]TJ
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4.5694 0 Td
([)Tj
0.83 0.64 0.02 0 k
(39)Tj
0 g
(].)Tj
-34.2025 -1.2982 Td
(Thus,)Tj
2.4718 0 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 246.0472 107.7165 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 251.7732 109.4173 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(can)Tj
1.627 0 Td
(be)Tj
1.1509 0 Td
(well)Tj
1.8198 0 Td
(reconstituted)Tj
5.4935 0 Td
(and)Tj
1.7291 0 Td
(studied)Tj
/F12 1 Tf
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1.7292 0 Td
(CD)Tj
-35.2684 -1.2982 Td
(and)Tj
1.7292 0 Td
(heme.)Tj
2.6418 0 Td
(Thus,)Tj
2.4718 0 Td
(the)Tj
1.4684 0 Td
(BC)Tj
1.4626 0 Td
(loop)Tj
1.9955 0 Td
(appears)Tj
3.2825 0 Td
(to)Tj
1.0261 0 Td
(not)Tj
1.5704 0 Td
(be)Tj
1.1509 0 Td
(crucial)Tj
2.914 0 Td
(for)Tj
1.3833 0 Td
(positioning)Tj
4.7792 0 Td
(or)Tj
1.0941 0 Td
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(the)Tj
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(the)Tj
1.4683 0 Td
(folding)Tj
3.0727 0 Td
(pathway.)Tj
3.7928 0 Td
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5.6181 0 Td
(removing)Tj
4.0762 0 Td
(any)Tj
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(of)Tj
1.0148 0 Td
(the)Tj
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4.3653 0 Td
(two)Tj
1.7121 0 Td
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(prote-)Tj
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(cleavage)Tj
3.5262 0 Td
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3.2769 0 Td
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1.7688 0 Td
(not)Tj
1.576 0 Td
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1.0261 0 Td
(affect)Tj
2.3754 0 Td
(the)Tj
1.4683 0 Td
(stability)Tj
3.3505 0 Td
(of)Tj
1.0148 0 Td
(cyt.)Tj
1.6271 0 Td
(b)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 429.5055 471.7417 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 433.0771 473.3858 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(.)Tj
0.4422 0 Td
(Likely,)Tj
2.8743 0 Td
(the)Tj
1.4683 0 Td
(holo-cytochrome)Tj
7.1149 0 Td
(can)Tj
-35.206 -1.2982 Td
(assemble)Tj
3.7928 0 Td
(solely)Tj
2.4604 0 Td
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2.2054 0 Td
(the)Tj
1.4683 0 Td
(TM)Tj
1.7235 0 Td
(regions)Tj
3.1634 0 Td
(and)Tj
1.7348 0 Td
(free)Tj
1.7235 0 Td
(heme.)Tj
2.6418 0 Td
(Thus,)Tj
2.4717 0 Td
(the)Tj
1.4684 0 Td
(soluble)Tj
3.0387 0 Td
(loop)Tj
1.9956 0 Td
(regions)Tj
3.1634 0 Td
(solely)Tj
-33.0517 -1.2983 Td
(guide)Tj
2.4322 0 Td
(and)Tj
1.7291 0 Td
(stabilize)Tj
3.4355 0 Td
(the)Tj
1.4684 0 Td
(assembly)Tj
3.8267 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(heme-binding)Tj
5.8961 0 Td
(TM)Tj
1.7234 0 Td
(four-helix)Tj
4.1895 0 Td
(bundle)Tj
3.0047 0 Td
(structure.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 421.1716 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Material)Tj
3.959 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F0 1 Tf
-5.948 -1.4976 Td
(Site-directed)Tj
5.7638 0 Td
(mutagenesis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 386.1921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Construction)Tj
5.5331 0 Td
(of)Tj
1.0092 0 Td
(the)Tj
1.4683 0 Td
(plasmid)Tj
3.3846 0 Td
(pRHMb)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 347.074 384.548 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 350.5889 386.1921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4422 0 Td
(coding)Tj
2.9536 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
/F12 1 Tf
1.4627 0 Td
[()14()11()12()6(\r)6()1()4(\r)]TJ
3.549 0 Td
[()10()4()2()20(\r)6()7()7(\r)]TJ
3.4072 0 Td
[()]TJ
/F7 1 Tf
0.7426 0 Td
(cyt.)Tj
1.6158 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 511.2 384.548 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 516.9259 386.1921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(protein,)Tj
3.3732 0 Td
(N-ter-)Tj
-35.0643 -1.2982 Td
(minally)Tj
3.2372 0 Td
(fused)Tj
2.347 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(maltose-binding)Tj
6.7918 0 Td
(protein)Tj
3.1521 0 Td
(\(MBP\))Tj
2.931 0 Td
(of)Tj
/F12 1 Tf
1.0148 0 Td
[( )]TJ
/F7 1 Tf
(.)Tj
/F12 1 Tf
0.9921 0 Td
[()7()10()12()]TJ
/F7 1 Tf
(,)Tj
1.7858 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(described)Tj
4.0534 0 Td
(previously)Tj
-32.4677 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
2.075 0 Td
(Mutation)Tj
3.9968 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 306.822 358.5259 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 312.6047 360.2267 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(amino)Tj
2.7949 0 Td
(acids)Tj
2.2337 0 Td
(His)Tj
1.6157 0 Td
(86)Tj
1.1736 0 Td
(and)Tj
1.7291 0 Td
(His)Tj
1.6157 0 Td
(187)Tj
1.6441 0 Td
(to)Tj
1.0261 0 Td
(alanine)Tj
3.1238 0 Td
(is)Tj
0.8447 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0375 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.5987 0 Td
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-35.7502 -1.3039 Td
(insertion)Tj
3.8097 0 Td
(of)Tj
1.0148 0 Td
(multiple)Tj
3.566 0 Td
(Gly)Tj
1.6384 0 Td
(residues)Tj
3.4808 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(BC-loop,)Tj
3.8154 0 Td
(an)Tj
1.2018 0 Td
(overlap)Tj
3.1748 0 Td
(extension)Tj
4.0649 0 Td
(polymerase)Tj
4.7792 0 Td
(chain)Tj
2.4207 0 Td
(reac-)Tj
-35.4724 -1.2982 Td
(tion)Tj
1.8425 0 Td
(\(PCR\))Tj
2.7439 0 Td
(was)Tj
1.6951 0 Td
(performed.)Tj
4.6942 0 Td
(In)Tj
1.1111 0 Td
(two)Tj
1.7064 0 Td
(separate)Tj
3.4866 0 Td
(PCRs,)Tj
2.6475 0 Td
(the)Tj
1.4684 0 Td
(gene)Tj
2.0806 0 Td
(was)Tj
1.7008 0 Td
(mutated)Tj
3.5489 0 Td
(in)Tj
1.0375 0 Td
(two)Tj
1.7064 0 Td
(partly)Tj
2.5455 0 Td
(comple-)Tj
-34.0154 -1.2983 Td
(mentary)Tj
3.5716 0 Td
(parts.)Tj
2.4265 0 Td
(The)Tj
1.7801 0 Td
(resulting)Tj
3.7303 0 Td
(PCR)Tj
2.0636 0 Td
(products)Tj
3.7531 0 Td
(overlap)Tj
3.1747 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(area)Tj
1.8822 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(newly)Tj
2.5795 0 Td
(inserted)Tj
3.4412 0 Td
(mutation.)Tj
-33.3918 -1.3039 Td
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1.7802 0 Td
(primers)Tj
3.3505 0 Td
(contained)Tj
4.2009 0 Td
(codons)Tj
3.0953 0 Td
(for)Tj
1.3833 0 Td
(Gly)Tj
1.6384 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.4683 0 Td
(5-ending)Tj
4.2179 0 Td
(and)Tj
1.7292 0 Td
(a)Tj
0.6576 0 Td
(3-ending)Tj
4.2179 0 Td
(complementary)Tj
6.5083 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
-36.2265 -1.2983 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 221.2724 293.5559 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 227.0551 295.2 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(coding)Tj
2.9593 0 Td
(sequence)Tj
3.8551 0 Td
(before)Tj
2.7439 0 Td
(or)Tj
1.0942 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4741 0 Td
(point)Tj
2.3584 0 Td
(of)Tj
1.0148 0 Td
(insertion)Tj
3.8097 0 Td
(\(between)Tj
3.8664 0 Td
(Pro)Tj
1.6611 0 Td
(113)Tj
1.6441 0 Td
(and)Tj
1.7291 0 Td
(Arg)Tj
1.7405 0 Td
(114\))Tj
-34.6901 -1.2982 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1)Tj
1.508 0 Td
(Table)Tj
0 g
(,)Tj
2.6702 0 Td
(No.)Tj
1.6894 0 Td
(14\).)Tj
2.2507 0 Td
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1.1112 0 Td
(a)Tj
0.6519 0 Td
(third)Tj
2.211 0 Td
(PCR,)Tj
2.2904 0 Td
(the)Tj
1.4684 0 Td
(3-ending)Tj
4.2235 0 Td
(of)Tj
1.0148 0 Td
(one)Tj
1.6952 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(products)Tj
3.753 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(first)Tj
1.8028 0 Td
(PCRs)Tj
-34.503 -1.3039 Td
(served)Tj
2.7722 0 Td
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1.0091 0 Td
(primer)Tj
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1.7235 0 Td
[()-13()4()7()]TJ
1.6724 0 Td
[()3()2()5(\n)-3(\r)]TJ
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(,)Tj
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(and)Tj
1.7234 0 Td
(thus)Tj
1.9276 0 Td
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(a)Tj
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1.4684 0 Td
(two)Tj
1.712 0 Td
(parts)Tj
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(of)Tj
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(the)Tj
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(mutated)Tj
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(gene)Tj
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1.6214 0 Td
(unmodified)Tj
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(primers)Tj
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(regions)Tj
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(coding)Tj
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(for)Tj
1.3833 0 Td
(the)Tj
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(N-)Tj
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(or)Tj
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1.0148 0 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 231.4204 228.5291 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 234.9921 230.1732 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4478 0 Td
(respectively)Tj
4.8813 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1)Tj
1.5136 0 Td
(Table)Tj
0 g
(,)Tj
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1.6894 0 Td
(56\).)Tj
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2.2847 0 Td
(restriction)Tj
4.354 0 Td
(digestion)Tj
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(by)Tj
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1.1792 0 Td
[()-6(\r)6()]TJ
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(I)Tj
1.8709 0 Td
(and)Tj
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1.7348 0 Td
[()-2(\r)16()]TJ
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(HI,)Tj
3.3845 0 Td
(the)Tj
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3.3902 0 Td
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1.0147 0 Td
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1.4684 0 Td
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2.2166 0 Td
(PCR)Tj
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(ligated)Tj
2.8856 0 Td
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1.8425 0 Td
(the)Tj
1.4684 0 Td
(equally)Tj
3.0557 0 Td
(restriction)Tj
4.3596 0 Td
(digested)Tj
3.515 0 Td
(vector)Tj
2.6986 0 Td
(pRHMb)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 549.8078 215.5464 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 553.3795 217.1905 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
-35.3364 -1.2982 Td
(resulting)Tj
3.7303 0 Td
(in)Tj
1.0319 0 Td
(the)Tj
1.4683 0 Td
(plasmids)Tj
3.7587 0 Td
(pRHMb)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 332.9574 202.507 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 336.4724 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(G)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 343.6724 202.507 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(5)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 349.3984 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(pRHMb)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 399.7984 202.507 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 403.3133 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(G)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 410.5133 202.507 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(10)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 417.6 204.2078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 178.0157 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Expression,)Tj
5.3858 0 Td
(purification)Tj
4.989 0 Td
(and)Tj
1.8662 0 Td
(reconstitution)Tj
6.0945 0 Td
(of)Tj
1.0441 0 Td
(cyt.)Tj
1.767 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
8.9997 0 0 7.999 460.3464 175.9748 cm
BT
/F0 1 Tf
1 TL
-0.0044 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 161.0078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Heterologous)Tj
5.6239 0 Td
(expression)Tj
4.4673 0 Td
(of)Tj
1.0148 0 Td
(cyt.)Tj
1.6214 0 Td
(b)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 332.3338 159.307 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 338.1165 161.0078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(in)Tj
/F12 1 Tf
1.0318 0 Td
[( )]TJ
/F7 1 Tf
(.)Tj
/F12 1 Tf
0.9977 0 Td
[()7()10()12()]TJ
/F7 1 Tf
1.5591 0 Td
(HMS174\(DE3\))Tj
6.2418 0 Td
(and)Tj
1.7292 0 Td
(subsequent)Tj
4.7054 0 Td
(purification)Tj
4.938 0 Td
(from)Tj
-35.0133 -1.2982 Td
(inclusion)Tj
3.9175 0 Td
(bodies,)Tj
3.0387 0 Td
(folding)Tj
3.0671 0 Td
(and)Tj
1.7291 0 Td
(reconstitution)Tj
5.8676 0 Td
(with)Tj
2.0012 0 Td
(heme)Tj
2.4095 0 Td
(for)Tj
1.3889 0 Td
(holo-protein)Tj
5.2895 0 Td
(formation)Tj
4.2632 0 Td
(were)Tj
2.1203 0 Td
(per-)Tj
-35.0926 -1.3039 Td
(formed)Tj
3.1578 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
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